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The Manuscript Collections contain family papers, diaries, church records, business records, papers of Kentucky authors, genealogical collections, 19th century manuscripts, historical materials relating to Kentucky and the Ohio River Valley region, and 20th century political collections of national importance. Major collections include:

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Envelope from a Civil War letter

1876 letter from C. J. Shoemaker to Colonel Simon S. Bowman from the Wade Hall Collection of American Letters.